Research highlight: new and powerful approach to wide-field fluorescence lifetime imaging with single-molecule sensitivity

Jörg Enderlein

doi:10.1186/s43593-025-00117-6

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Research highlight: new and powerful approach to wide-field fluorescence lifetime imaging with single-molecule sensitivity

eLight Vol. 5, (2025)

Editorial | Updated：2025-12-17

- Research highlight: new and powerful approach to wide-field fluorescence lifetime imaging with single-molecule sensitivity
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- DOI：10.1186/s43593-025-00117-6
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- Published Online：10 December 2025，
  
  Published：2025-12
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Jörg Enderlein. Research highlight: new and powerful approach to wide-field fluorescence lifetime imaging with single-molecule sensitivity[J]. eLight, 2025, 5.
DOI：

Jörg Enderlein. Research highlight: new and powerful approach to wide-field fluorescence lifetime imaging with single-molecule sensitivity[J]. eLight, 2025, 5. DOI： 10.1186/s43593-025-00117-6.

摘要

Abstract

Historically constrained by the low throughput of traditional Time-Correlated Single-Photon Counting (TCSPC)

a new method utilizes an optimized

time-gated single-photon camera acquisition scheme for achieving single-molecule lifetime precision comparable to TCSPC

but across a wide field of view simultaneously. This high-throughput capability transforms single-molecule FLIM from a specialized

time-consuming effort into a scalable

quantitative assay. The resulting multimodal imaging platform provides unprecedented simultaneous insight into the nanoscale location (SMLM) and local environment (FLIM) of biological molecules

advancing our ability to map complex biophysical parameters like FRET efficiency in living systems.

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references

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