Light-driven single-cell rotational adhesion frequency assay

Yaoran Liu; Hongru Ding; Jingang Li; Xin Lou; Mingcheng Yang; Yuebing Zheng

doi:10.1186/s43593-022-00020-4

Your Location：

Home >

Browse articles >

Light-driven single-cell rotational adhesion frequency assay

eLight Vol. 2, Issue 1, (2022)

RESEARCH ARTICLE | Updated：2024-09-28

- Light-driven single-cell rotational adhesion frequency assay
- Affiliations：
  
  Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, TX 78712, USA
  Walker Department of Mechanical Engineering, The University of Texas at Austin, Austin, TX 78712, USA
  Materials Science& Engineering Program and Texas Materials Institute, The University of Texas at Austin, Austin, TX 78712, USA
  School of Physical Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
  Beijing National Laboratory for Condensed Matter Physics and Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China
  Songshan Lake Materials Laboratory, Dongguan 523808, Guangdong, China
  Department of Biomedical Engineering, The University of Texas at Austin,Austin, TX 78712, USA
- Author bio：
  
  *Yuebing Zhengzheng@austin.utexas.edu
- Funds：
- DOI：10.1186/s43593-022-00020-4
  CLC：
- Published：2022-12，
  
  Published Online：08 August 2022，
  
  Received：24 May 2022，
  
  Revised：28 June 2022，
  
  Accepted：07 July 2022
- Accepted：
Scan QR Code
Yaoran Liu, Hongru Ding, Jingang Li, et al. Light-driven single-cell rotational adhesion frequency assay. [J]. eLight, 2022,2(1).
DOI：

Yaoran Liu, Hongru Ding, Jingang Li, et al. Light-driven single-cell rotational adhesion frequency assay. [J]. eLight, 2022,2(1). DOI： 10.1186/s43593-022-00020-4.

摘要

Abstract

The interaction between cell surface receptors and extracellular ligands is highly related to many physiological processes in living systems. Many techniques have been developed to measure the ligand-receptor binding kinetics at the single-cell level. However

few techniques can measure the physiologically relevant shear binding affinity over a single cell in the clinical environment. Here

we develop a new optical technique

termed single-cell rotational adhesion frequency assay (scRAFA)

that mimics in vivo cell adhesion to achieve label-free determination of both homogeneous and heterogeneous binding kinetics of targeted cells at the subcellular level. Moreover

the scRAFA is also applicable to analyze the binding affinities on a single cell in native human biofluids. With its superior performance and general applicability

scRAFA is expected to find applications in study of the spatial organization of cell surface receptors and diagnosis of infectious diseases.

关键词

Keywords

references

S.M. Albelda, C.A. Buck, Integrins and other cell adhesion molecules. FASEB J. 4, 2868-2880 (1990)

B.M. Gumbiner, Cell adhesion: the molecular basis of tissue architecture and morphogenesis. Cell 84, 345-357 (1996)

D. Vestweber, How leukocytes cross the vascular endothelium. Nat. Rev.Immunol. 15, 692-704 (2015)

T.R. Garrett, M. Bhakoo, Z. Zhang, Bacterial adhesion and biofilms on surfaces. Prog. Nat. Sci. 18, 1049-1056 (2008)

A. Iwasaki, P.S. Pillai, Innate immunity to influenza virus infection. Nat. Rev.Immunol. 14, 315-328 (2014)

K. Ley, C. Laudanna, M.I. Cybulsky, S. Nourshargh, Getting to the site of inflammation: the leukocyte adhesion cascade updated. Nat. Rev. Immunol. 7, 678-689 (2007)

K.C. Neuman, A. Nagy, Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy. Nat. Methods 5, 491-505 (2008)

S.E. Chesla, P. Selvaraj, C. Zhu, Measuring two-dimensional receptorligand binding kinetics by micropipette. Biophys. J. 75, 1553-1572 (1998)

W.R. Legant et al., Measurement of mechanical tractions exerted by cells in three-dimensional matrices. Nat. Methods 7, 969 (2010)

P. Hinterdorfer, Y.F. Dufrêne, Detection and localization of single molecular recognition events using atomic force microscopy. Nat. Methods 3,347-355 (2006)

Y. Zhang, G. Sun, S. Lü, N. Li, M. Long, Low spring constant regulates P-selectin-PSGL-1 bond rupture. Biophys. J. 95, 5439-5448 (2008)

D. Wrapp et al., Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science 367, 1260-1263 (2020)

J. Yang et al., Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor. Nat. Comm. 11, 1-10 (2020)

R.P. McEver, C. Zhu, Rolling cell adhesion. Annu. Rev. Cell Dev. Biol. 26,363-396 (2010)

C. Zhu, W. Chen, J. Lou, W. Rittase, K. Li, Mechanosensing through immunoreceptors. Nat. Immunol. 20, 1269-1278 (2019)

A. Katsumi, A.W. Orr, E. Tzima, M.A. Schwartz, Integrins in mechanotransduction. J. Biol. Chem. 279, 12001-12004 (2004)

R. Alon, D.A. Hammer, T.A. Springer, Lifetime of the P-selectin-carbohydrate bond and its response to tensile force in hydrodynamic flow. Nature 374, 539-542 (1995)

K.-C. Chang, D.F. Tees, D.A. Hammer, The state diagram for cell adhesion under flow: leukocyte rolling and firm adhesion. Proc. Natl. Acad. Sci. 97,11262-11267 (2000)

A.B. Stilgoe, T.A. Nieminen, H. Rubinsztein-Dunlop, Controlled transfer of transverse orbital angular momentum to optically trapped birefringent microparticles. Nat. Photonics 16, 346-351 (2022)

M.E. Friese, T.A. Nieminen, N.R. Heckenberg, H. Rubinsztein-Dunlop, Optical alignment and spinning of laser-trapped microscopic particles. Nature 394, 348-350 (1998)

A. Grigorenko, N. Roberts, M. Dickinson, Y. Zhang, Nanometric optical tweezers based on nanostructured substrates. Nat. Photonics 2, 365-370(2008)

P.Y. Chiou, A.T. Ohta, M.C. Wu, Massively parallel manipulation of single cells and microparticles using optical images. Nature 436, 370-372(2005)

Y. Pang, R. Gordon, Optical trapping of a single protein. Nano Lett. 12,402-406 (2012)

L. Lin, E.H. Hill, X. Peng, Y. Zheng, Optothermal manipulations of colloidal particles and living cells. Acc. Chem. Res. 51, 1465-1474 (2018)

A.P. Bregulla, A. Würger, K. Günther, M. Mertig, F. Cichos, Thermo-osmotic flow in thin films. Phys. Rev. Lett. 116, 188303 (2016)

M. Fränzl et al., Thermophoretic trap for single amyloid fibril and protein aggregation studies. Nat. Meth. 16, 611-614 (2019)

X. Lou, N. Yu, R. Liu, K. Chen, M. Yang, Dynamics of a colloidal particle near a thermoosmotic wall under illumination. Soft Matter 14, 1319-1326(2018)

S. Kumar et al., Pitch-rotational manipulation of single cells and particles using single-beam thermo-optical tweezers. Biomed. Opt. Express 11,3555-3566 (2020)

L. Hackett, S. Seo, S. Kim, L. Goddard, G. Liu, Label-free cell-substrate adhesion imaging on plasmonic nanocup arrays. Biomed. Opt. Express 8,1139-1151 (2017)

F. Ahimou, A. Touhami, Y.F. Dufrêne, Real-time imaging of the surface topography of living yeast cells by atomic force microscopy. Yeast 20,25-30 (2003)

S. El-Kirat-Chatel et al., Single-molecule analysis of the major glycopolymers of pathogenic and non-pathogenic yeast cells. Nanoscale 5,4855-4863 (2013)

A.D. McCall, R.U. Pathirana, A. Prabhakar, P.J. Cullen, M. Edgerton, Candida albicans biofilm development is governed by cooperative attachment and adhesion maintenance proteins. npj Biofilms Microbiomes 5, 1-12(2019)

M. Schiavone et al., AFM dendritips functionalized with molecular probes specific to cell wall polysaccharides as a tool to investigate cell surface structure and organization. Cell Surface 5, 100027 (2019)

A. Touhami, B. Nysten, Y.F. Dufrêne, Nanoscale mapping of the elasticity of microbial cells by atomic force microscopy. Langmuir 19, 4539-4543(2003)

P.H. Leliefeld, L. Koenderman, J. Pillay, How neutrophils shape adaptive immune responses. Front. Immunol. 6, 471 (2015)

L.J. Jones, R. Carballido-López, J. Errington, Control of cell shape in bacteria: helical, actin-like filaments in Bacillus subtilis. Cell 104, 913-922 (2001)

J. Haiko, B. Westerlund-Wikström, The role of the bacterial flagellum in adhesion and virulence. Biology 2, 1242-1267 (2013)

P. Reichert, R.L. Reinhardt, E. Ingulli, M.K. Jenkins, Cutting edge: in vivo identification of TCR redistribution and polarized IL-2 production by naive CD4 T cells. J. Immunol. Res. 166, 4278-4281 (2001)

X. Ding et al., High-throughput nuclear delivery and rapid expression of DNA via mechanical and electrical cell-membrane disruption. Nat.Biomed. Eng. 1, 1-7 (2017)

P. Behzadi, E. Behzadi, R. Ranjbar, Urinary tract infections and Candida albicans. Curr. Opin. Struct. Biol. 68, 96 (2015)

C.F. Brewer, M.C. Miceli, L.G. Baum, Clusters, bundles, arrays and lattices:novel mechanisms for lectin–saccharide-mediated cellular interactions. Curr. Opin. Struct. Biol. 12, 616-623 (2002)

J. Huang et al., The kinetics of two-dimensional TCR and pMHC interactions determine T-cell responsiveness. Nature 464, 932-936 (2010)

W.E. Thomas, L.M. Nilsson, M. Forero, E.V. Sokurenko, V. Vogel, Sheardependent 'stick-and-roll' adhesion of type 1 fimbriated Escherichia coli. Mol. Microbiol. 53, 1545-1557 (2004)

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰