A Through-Intact-Skull (TIS) chronic window technique for cortical structure and function observation in mice

Dongyu Li; Zhengwu Hu; Hequn Zhang; Qihang Yang; Liang Zhu; Yin Liu; Tingting Yu; Jingtan Zhu; Jiamin Wu; Jing He; Peng Fei; Wang Xi; Jun Qian; Dan Zhu

doi:10.1186/s43593-022-00022-2

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A Through-Intact-Skull (TIS) chronic window technique for cortical structure and function observation in mice

eLight Vol. 2, Issue 1, (2022)

RESEARCH ARTICLE | Updated：2024-09-28

- A Through-Intact-Skull (TIS) chronic window technique for cortical structure and function observation in mice
- Affiliations：
  
  Britton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics - Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China
  Optics Valley Laboratory, Hubei 430074, China
  State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou 310058, China
  Interdisciplinary Institute of Neuroscience and Technology, Department of Anesthesiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Kaixuan Road 258th, Hangzhou 310020, China
  Department of Automation, Tsinghua University, Beijing 100084, China
  School of Optical Electronic Information -Wuhan National Laboratory for Optoelectronics - Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China
- Author bio：
  
  *xw333@zju.edu.cn
  qianjun@zju.edu.cn
  dawnzh@mail.hust.edu.cn
- Funds：
- DOI：10.1186/s43593-022-00022-2
  CLC：
- Published：2022-12，
  
  Published Online：05 September 2022，
  
  Received：20 June 2022，
  
  Revised：03 August 2022，
  
  Accepted：04 August 2022
- Accepted：
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Dongyu Li, Zhengwu Hu, Hequn Zhang, et al. A Through-Intact-Skull (TIS) chronic window technique for cortical structure and function observation in mice. [J]. eLight, 2022,2(1).
DOI：

Dongyu Li, Zhengwu Hu, Hequn Zhang, et al. A Through-Intact-Skull (TIS) chronic window technique for cortical structure and function observation in mice. [J]. eLight, 2022,2(1). DOI： 10.1186/s43593-022-00022-2.

摘要

Abstract

Modern optical imaging techniques provide powerful tools for observing cortical structure and functions at high resolutions. Various skull windows have been established for different applications of cortical imaging

and each has its advantages and limitations. Most critical of the limitations

none of the current skull windows is suitable for observing the responses to some acute craniocerebral injuries on a large scale and at high resolution. Here

we developed a "Through-Intact-Skull (TIS) window" that enables the observation of an immune response on a bilateral cortical scale and at single-cell resolution after traumatic brain injury without affecting the pathological environment of the brain. The TIS window also has the advantages of craniotomy-freeness

centimeter-field of view

synaptic resolution

large imaging depth

long-term observation capability

and suitability for awake mice. Therefore

the TIS window is a promising new approach for intravital cortical microscopy in basic research in neuroscience.

关键词

Keywords

Skull optical clearing windowLong-term neurovascular imagingTraumatic brain injuryAwake animal

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