Upconversion nanoparticles for super-resolution quantification of single small extracellular vesicles

Guan Huang; Yongtao Liu; Dejiang Wang; Ying Zhu; Shihui Wen; Juanfang Ruan; Dayong Jin

doi:10.1186/s43593-022-00031-1

Your Location：

Home >

Browse articles >

Upconversion nanoparticles for super-resolution quantification of single small extracellular vesicles

eLight Vol. 2, Issue 1, (2022)

RESEARCH ARTICLE | Updated：2024-09-28

- Upconversion nanoparticles for super-resolution quantification of single small extracellular vesicles
- Affiliations：
  
  Institute for Biomedical Materials and Devices (IBMD), Faculty of Science,University of Technology Sydney, Sydney, NSW 2007, Australia
  Smart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China
  School of Biomedical Engineering, Faculty of Engineering and Information Technology, University of Technology Sydney, Sydney, NSW 2007, Australia
  School of Clinical Medicine, Faculty of Medicine & Health, UNSW Sydney, Sydney, NSW 2052, Australia
  Electron Microscope Unit, UNSW Sydney, Sydney, NSW 2052, Australia
  ARC Research Hub for Integrated Device for End-User Analysis at Low Levels, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia
  UTS-SUStech Joint Research Centre for Biomedical Materials and Devices, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China
- Author bio：
  
  *Dayong Jindayong.jin@uts.edu.au
- Funds：
- DOI：10.1186/s43593-022-00031-1
  CLC：
- Published：2022-12，
  
  Published Online：10 October 2022，
  
  Received：04 August 2022，
  
  Revised：10 September 2022，
  
  Accepted：16 September 2022
- Accepted：
Scan QR Code
Guan Huang, Yongtao Liu, Dejiang Wang, et al. Upconversion nanoparticles for super-resolution quantification of single small extracellular vesicles. [J]. eLight, 2022,2(1).
DOI：

Guan Huang, Yongtao Liu, Dejiang Wang, et al. Upconversion nanoparticles for super-resolution quantification of single small extracellular vesicles. [J]. eLight, 2022,2(1). DOI： 10.1186/s43593-022-00031-1.

摘要

Abstract

Although small EVs (sEVs) have been used widely as biomarkers in disease diagnosis

their heterogeneity at single EV level has rarely been revealed. This is because high-resolution characterization of sEV presents a major challenge

as their sizes are below the optical diffraction limit. Here

we report that upconversion nanoparticles (UCNPs) can be used for super-resolution profiling the molecular heterogeneity of sEVs. We show that Er

-doped UCNPs has better brightness and Tm

-doped UCNPs resulting in better resolution beyond diffraction limit. Through an orthogonal experimental design

the specific targeting of UCNPs to the tumour epitope on single EV has been cross validated

resulting in the Pearson's R-value of 0.83 for large EVs and ~ 65% co-localization double-positive spots for sEVs. Furthermore

super-resolution nanoscopy can distinguish adjacent UCNPs on single sEV with a resolution of as high as 41.9 nm. When decreasing the size of UCNPs from 40 to 27 nm and 18 nm

we observed that the maximum UCNPs number on single sEV increased from 3 to 9 and 21

respectively. This work suggests the great potentials of UCNPs approach "digitally" quantify the surface antigens on single EVs

therefore providing a solution to monitor the EV heterogeneity changes along with the tumour progression progress.

关键词

Keywords

Extracellular vesiclesUpconversion nanoparticlesSuper-resolutionLiquid biopsy

references

G. Bergers, S.-M. Fendt, Nat. Rev. Cancer 21, 162-180 (2021)

Editorial, Nat. Biomed. Eng. 1, 925 (2017)

L.R. Yates, M. Gerstung, S. Knappskog, C. Desmedt, G. Gundem, P. Van Loo,T. Aas, L.B. Alexandrov, D. Larsimont, H. Davies, Y. Li, Y.S. Ju, M. Ramakrishna, H.K. Haugland, P.K. Lilleng, S. Nik-Zainal, S. McLaren, A. Butler, S. Martin, D. Glodzik, A. Menzies, K. Raine, J. Hinton, D. Jones, L.J. Mudie, B. Jiang, D. Vincent, A. Greene-Colozzi, P.-Y. Adnet, A. Fatima, M. Maetens,M. Ignatiadis, M.R. Stratton, C. Sotiriou, A.L. Richardson, P.E. Lønning, D.C. Wedge, P.J. Campbell, Nat. Med. 21, 751-759 (2015)

M. Tkach, C. Théry, Cell 164, 1226-1232 (2016)

A. Möller, R.J. Lobb, Nat. Rev. Cancer 20, 697-709 (2020)

R. Xu, A. Rai, M. Chen, W. Suwakulsiri, D.W. Greening, R.J. Simpson, Nat.Rev. Clin. Oncol. 15, 617-638 (2018)

S.T.-Y. Chuo, J.C.-Y. Chien, C.P.-K. Lai, J. Biomed. Sci. 25, 91 (2018)

G. Huang, G. Lin, Y. Zhu, W. Duan, D. Jin, Lab. Chip. 20, 2423-2437 (2020)

Z. Nizamudeen, R. Markus, R. Lodge, C. Parmenter, M. Platt, L. Chakrabarti,V. Sottile, Biochim. Biophys. Acta. Mol. Cell Res. 2018, 1891-1900 (1865)

C. Han, H. Kang, J. Yi, M. Kang, H. Lee, Y. Kwon, J. Jung, J. Lee, J. Park, J. Extracell, Vesicles 10, e12047 (2021)

R.R. Iyer, J.E. Sorrells, L. Yang, E.J. Chaney, D.R. Spillman, B.E. Tibble, C.A. Renteria, H. Tu, M. Žurauskas, M. Marjanovic, S.A. Boppart, Sci. Rep. 12,3438 (2022)

K. Burbidge, V. Zwikelmaier, B. Cook, M.M. Long, B. Balva, M. Lonigro, G. Ispas, D.J. Rademacher, E.M. Campbell, J. Extracell, Vesicles 9, 1789326(2020)

G. Corso, W. Heusermann, D. Trojer, A. Görgens, E. Steib, J. Voshol, A. Graff,C. Genoud, Y. Lee, J. Hean, J.Z. Nordin, O.P.B. Wiklander, S. El Andaloussi, N. Meisner-Kober, J. Extracell. Vesicles 8, 1663043 (2019)

G. Bordanaba-Florit, F. Royo, S.G. Kruglik, J.M. Falcón-Pérez, Nat. Protoc. 16,3163-3185 (2021)

L. Schermelleh, A. Ferrand, T. Huser, C. Eggeling, M. Sauer, O. Biehlmaier,G.P.C. Drummen, Nat. Cell Biol. 21, 72-84 (2019)

J.C. Polanco, C. Li, N. Durisic, R. Sullivan, J. Götz, Acta Neuropathol. Commun. 6, 10 (2018)

S. Zong, J. Zong, C. Chen, X. Jiang, Y. Zhang, Z. Wang, Y. Cui, Nanotechnology 29, 65705 (2018)

Y. Jiang, L.A. Andronico, S.-R. Jung, H. Chen, B. Fujimoto, L. Vojtech, D.T. Chiu, Angew. Chemie Int. Ed. 60, 13470-13475 (2021)

C. Chen, S. Zong, Z. Wang, J. Lu, D. Zhu, Y. Zhang, Y. Cui, A.C.S. Appl, Mater.Interfaces 8, 25825-25833 (2016)

R.P. McNamara, Y. Zhou, A.B. Eason, J.T. Landis, M.G. Chambers, S. Willcox,T.A. Peterson, B. Schouest, N.J. Maness, A.G. MacLean, L.M. Costantini, J.D. Griffith, D.P. Dittmer, J. Extracell, Vesicles 11, e12191 (2022)

Z. Nizamudeen, R. Markus, R. Lodge, C. Parmenter, M. Platt, L. Chakrabarti,V. Sottile, Biochim. Biophys. Acta. Mol. cell Res. 2018, 1891-1900 (1865)

Y. Liu, Y. Lu, X. Yang, X. Zheng, S. Wen, F. Wang, X. Vidal, J. Zhao, D. Liu, Z. Zhou, C. Ma, J. Zhou, J.A. Piper, P. Xi, D. Jin, Nature 543, 229-233 (2017)

Q. Zhan, H. Liu, B. Wang, Q. Wu, R. Pu, C. Zhou, B. Huang, X. Peng, H. Ågren,S. He, Nat. Commun. 8, 1058 (2017)

Y. Liang, Z. Zhu, S. Qiao, X. Guo, R. Pu, H. Tang, H. Liu, H. Dong, T. Peng, L.-D. Sun, J. Widengren, Q. Zhan, Nat. Nanotechnol. 17, 524-530 (2022)

X. Guo, R. Pu, Z. Zhu, S. Qiao, Y. Liang, B. Huang, H. Liu, L. Labrador-Páez,U. Kostiv, P. Zhao, Q. Wu, J. Widengren, Q. Zhan, Nat. Commun. 13, 2843(2022)

D. Denkova, M. Ploschner, M. Das, L.M. Parker, X. Zheng, Y. Lu, A. Orth, N.H. Packer, J.A. Piper, Nat. Commun. 10, 3695 (2019)

C. Chen, F. Wang, S. Wen, Q.P. Su, M.C.L. Wu, Y. Liu, B. Wang, D. Li, X. Shan,M. Kianinia, I. Aharonovich, M. Toth, S.P. Jackson, P. Xi, D. Jin, Nat. Commun. 9, 3290 (2018)

L. Liang, Z. Feng, Q. Zhang, T. Do Cong, Y. Wang, X. Qin, Z. Yi, M.J.Y. Ang, L. Zhou, H. Feng, B. Xing, M. Gu, X. Li, X. Liu, Nat. Nanotechnol. 16, 975-980(2021)

G. Huang, Y. Zhu, S. Wen, H. Mei, Y. Liu, D. Wang, M. Maddahfar, Q.P. Su, G. Lin, Y. Chen, D. Jin, Nano Lett. 22, 3761-3769 (2022)

S.W. Hell, Nat. Biotechnol. 21, 1347-1355 (2003)

G. Zhao, C. Kuang, Z. Ding, X. Liu, Opt. Express 24, 23596-23609 (2016)

Q. Su, H.-L. Wei, Y. Liu, C. Chen, M. Guan, S. Wang, Y. Su, H. Wang, Z. Chen,D. Jin, Nat. Commun. 12, 4367 (2021)

P. Amrollahi, M. Rodrigues, C. J. Rodrigues, A. Goel, H. Han and T. Y. Hu,Front. Genet. 10, 1273 (2019)

E.M.M. Manders, F.J. Verbeek, J.A. Aten, J. Microsc. 169, 375-382 (1993)

C. Théry, K.W. Witwer, E. Aikawa, E. Alcaraz, J. Extracell, Vesicles 7, 1535750(2018)

S. Wen, J. Zhou, K. Zheng, A. Bednarkiewicz, X. Liu, D. Jin, Nat. Commun. 9,2415 (2018)

A. Emelyanov, T. Shtam, R. Kamyshinsky, L. Garaeva, N. Verlov, I. Miliukhina,A. Kudrevatykh, G. Gavrilov, Y. Zabrodskaya, S. Pchelina, A. Konevega, PLoS ONE 15, e0227949 (2020)

D. Zabeo, A. Cvjetkovic, C. Lässer, M. Schorb, J. Lötvall, J.L. Höög, J. Extracell, Vesicles 6, 1329476 (2017)

Z. Andreu, E. Rivas, A. Sanguino-Pascual, A. Lamana, M. Marazuela, I. González-Alvaro, F. Sánchez-Madrid, H. de la Fuente, M. Yáñez-Mó, J.Extracell. Vesicles 5, 31655 (2016)

D.J. Gargas, E.M. Chan, A.D. Ostrowski, S. Aloni, M.V.P. Altoe, E.S. Barnard, B. Sanii, J.J. Urban, D.J. Milliron, B.E. Cohen, P.J. Schuck, Nat. Nanotechnol. 9,300-305 (2014)

C. Liu, J. Zhao, F. Tian, L. Cai, W. Zhang, Q. Feng, J. Chang, F. Wan, Y. Yang, B. Dai, Y. Cong, B. Ding, J. Sun, W. Tan, Nat. Biomed. Eng. 3, 183-193 (2019)

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Publicity Resources

Structured illumination microscopy based on principal component analysis

Related Author

Jiaming Qian

Yu Cao

Ying Bi

Hongjun Wu

Yongtao Liu

Qian Chen

Chao Zuo

Related Institution

Smart Computational Imaging (SCI) Laboratory (https://scilaboratory.com/), Nanjing University of Science and Technology, No. Xiaolingwei Street

Smart Computational Imaging Research Institute (SCIRI) of Nanjing University of Science and Technology

Jiangsu Key Laboratory of Spectral Imaging & Intelligent Sense, No. Xiaolingwei Street

⁰