ISO-FLUCS: symmetrization of optofluidic manipulations in quasi-isothermal micro-environments

Antonio Minopoli; Susan Wagner; Elena Erben; Weida Liao; Iliya D. Stoev; Eric Lauga; Moritz Kreysing

doi:10.1186/s43593-023-00049-z

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ISO-FLUCS: symmetrization of optofluidic manipulations in quasi-isothermal micro-environments

eLight Vol. 3, Issue 1, (2023)

RESEARCH ARTICLE | Updated：2024-09-25

- ISO-FLUCS: symmetrization of optofluidic manipulations in quasi-isothermal micro-environments
- Affiliations：
  
  Max-Planck-Institute of Molecular Cell Biology and Genetics, Dresden, Germany
  Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Cambridge, UK
  Center for Systems Biology Dresden, Dresden, Germany
  Institute of Biological and Chemical Systems-Biological Information Processing, Karlsruhe Institute of Technology, Karlsruhe, Germany
- Author bio：
  
  *Moritz Kreysingmoritz.kreysing@kit.edu
- Funds：
- DOI：10.1186/s43593-023-00049-z
  CLC：
- Published：2023，
  
  Published Online：18 July 2023，
  
  Received：16 March 2023，
  
  Revised：10 May 2023，
  
  Accepted：24 May 2023
- Accepted：
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Antonio Minopoli, Susan Wagner, Elena Erben, et al. ISO-FLUCS: symmetrization of optofluidic manipulations in quasi-isothermal micro-environments. [J]. eLight, 2023,3(1).
DOI：

Antonio Minopoli, Susan Wagner, Elena Erben, et al. ISO-FLUCS: symmetrization of optofluidic manipulations in quasi-isothermal micro-environments. [J]. eLight, 2023,3(1). DOI： 10.1186/s43593-023-00049-z.

摘要

Abstract

Recently

it has been demonstrated that thermoviscous flows can be used for a range of fine micromanipulations

such as moving the cytoplasm of cells and developing embryos

intracellular rheology

and femtonewton-range force measurements. These flows

also known as focused-light-induced cytoplasmic streaming (FLUCS)

are induced by mid-infrared laser scanning of a temperature spot through the sample. However

localized laser scanning can inflict temperature perturbations of several Kelvins on the sample

potentially eliciting unspecific biological responses. In this study

we demonstrate how exploiting symmetry relations during laser scanning effectively disentangles laser heating and flow induction. We introduce flow-neutral scan sequences that use dynamic photothermal stimuli and spatiotemporal symmetry relations of scanning bridging up to three distinct time scales. We leverage further insights from a recently published analytical model of flow fields to present quasi-homogenous temperature distributions that leave flow lines and their local and directed character largely invariant. We present practical

intuitive solutions through predesigned sets of scan lines with near isothermal distributions and demonstrate that they are sufficient to generate and control flows in

Caenorhabditis elegans

embryos on a magnitude well in excess of endogenous flow velocities. Our results enable the separation of two previously tightly linked classes of physical stimuli

introduce a new

even less invasive standard for performing FLUCS perturbations

and pave the way for new unexplored avenues in the fields of soft matter and biomedicine.

关键词

Keywords

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