Light-driven single-cell rotational adhesion frequency assay

Yaoran Liu; Hongru Ding; Jingang Li; Xin Lou; Mingcheng Yang; Yuebing Zheng

doi:10.1186/s43593-022-00020-4

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Light-driven single-cell rotational adhesion frequency assay

光：快讯（英文） 2022年2卷第1期

Research Article | Updated：2024-09-28

- Light-driven single-cell rotational adhesion frequency assay
- Light-driven single-cell rotational adhesion frequency assay
- Affiliations：
  
  Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, TX 78712, USA
  Walker Department of Mechanical Engineering, The University of Texas at Austin, Austin, TX 78712, USA
  Materials Science& Engineering Program and Texas Materials Institute, The University of Texas at Austin, Austin, TX 78712, USA
  School of Physical Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
  Beijing National Laboratory for Condensed Matter Physics and Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China
  Songshan Lake Materials Laboratory, Dongguan 523808, Guangdong, China
  Department of Biomedical Engineering, The University of Texas at Austin,Austin, TX 78712, USA
- Author bio：
  
  *Yuebing Zhengzheng@austin.utexas.edu
- Funds：
  
  National Institute of General Medical Sciences of the National Institutes of Health(DP2GM128446);National Science Foundation(ECCS-2001650);National Natural Science Foundation of China(11874397)
- DOI：10.1186/s43593-022-00020-4
  中图分类号：
- 纸质出版日期：2022-12，
  
  网络出版日期：2022-08-08，
  
  收稿日期：2022-05-24，
  
  修回日期：2022-06-28，
  
  录用日期：2022-07-07
- Accepted：
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Light-driven single-cell rotational adhesion frequency assay[J]. 光：快讯（英文）, 2022,2(1).

Yaoran Liu, Hongru Ding, Jingang Li, et al. Light-driven single-cell rotational adhesion frequency assay[J]. eLight, 2022,2(1).
Light-driven single-cell rotational adhesion frequency assay[J]. 光：快讯（英文）, 2022,2(1). DOI： 10.1186/s43593-022-00020-4.

Yaoran Liu, Hongru Ding, Jingang Li, et al. Light-driven single-cell rotational adhesion frequency assay[J]. eLight, 2022,2(1). DOI： 10.1186/s43593-022-00020-4.

摘要

Abstract

The interaction between cell surface receptors and extracellular ligands is highly related to many physiological processes in living systems. Many techniques have been developed to measure the ligand-receptor binding kinetics at the single-cell level. However

few techniques can measure the physiologically relevant shear binding affinity over a single cell in the clinical environment. Here

we develop a new optical technique

termed single-cell rotational adhesion frequency assay (scRAFA)

that mimics in vivo cell adhesion to achieve label-free determination of both homogeneous and heterogeneous binding kinetics of targeted cells at the subcellular level. Moreover

the scRAFA is also applicable to analyze the binding affinities on a single cell in native human biofluids. With its superior performance and general applicability

scRAFA is expected to find applications in study of the spatial organization of cell surface receptors and diagnosis of infectious diseases.

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references

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